Detection of dna-containing extra cellular vesicles in the supernatant of cultured embryos using facs to increase the efficiency of in vitro fertilization

摘要

The invention relates to a non-invasive, simple, inexpensive, quick method to assess embryo competence in vitro by detecting nucleic acid-containing extracellular vesicles in the culture medium of the embryo. The culture medium may be fixed before being stained with propidium iodide and analysed by flow cytometry.