Expanded bed adsorption methods for isolation of basic milk proteins including lactoferrin

摘要

The present disclosure relates to improved expanded bed adsorption processes for isolating lactoferrin from milk sources. The process comprising establishing an expanded bed adsorption column comprising a particulate matrix, adding citrates to a diafiltered milk source, pre-eluting lactoperoxidase with 0.2-0.3 M NaCl and then eluting lactoferrin by 0.3-2.0 M NaCl at 30-50°C.