Recombinant bacterium for synthesizing odd and even carbon chain monomer copolymerized medium-chain-length polyhydroxyalkanoates

摘要

The invention provides a recombinant plasmid pZQ12, a recombinant strain for synthetizing odevity carbon-chain monomer copolymerization mcl-PHA and an application of the recombinant strain, and belongs to the technical field of genetic engineering. The recombinant plasmid pZQ12 comprises that cimA genes are inserted into NcoI and BamHI multiple clone sites of a plasmid pACYCDuet-1, leuBCD genes are inserted into BamHI and SacI multiple clone sites of the plasmid pACYCDuet-1, ilvA genes are inserted into SacI and AscI multiple clone sites of the plasmid pACYCDuet-1 and thrA*BC genes are inserted into AscI multiple clone sites of the plasmid pACYCDuet-1. The recombinant plasmid pQQ05 of the recombinant plasmid pZQ12 is transferred to escherichia coli LZ09 to obtain the recombinant strain. Glucose which is cheap and broad in source is used as the only carbon source, and the odevity carbon-chain monomer copolymerization mcl-PHA can be synthetized.