METHODS AND REAGENTS FOR DETECTING AND ASSESSING GENOTOXICITY

摘要

Methods, systems, and kits with reagents for assessing genotoxicity, are disclosed herein. Genotoxicity and their mechanisms of action can be determined within a few days of a subject's exposure. Some embodiments of the technology are directed to utilizing Duplex Sequencing for assessing a genotoxic potential of a compound (e.g., a chemical compound) in an exposed subject. Other embodiments of the technology are directed to utilizing Duplex Sequencing for determining a mutation signature associated with a genotoxic agent; and/or a safe threshold level of genotoxin exposure. Additional embodiments of the technology are directed to identifying one or more genotoxic agents a subject may have been exposed to by comparing the subject's DNA mutation spectrum to the mutation spectra of known mutagenic compounds. Once a genotoxin exposure in a subject is identified, or confirmed, then a prophylactic, and/or inhibitory therapeutic course of treatment is provided.