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Capture of directed chromosomal conformation
Capture of directed chromosomal conformation
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机译:捕获定向染色体构象
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摘要
The invention relates to a method which enables the possibility to capture chromosome conformation as well as a kit containing components useful to be used the method, such as to detect promoter and enhancer relations. The method comprises the following steps: i) providing cross-linked genomic DNA, wherein the DNA comprises a first and a second set of regions, ii) fragmenting the cross-linked genome, thus creating a plurality of fragments with junctions, iii) adding a labelled junction marker such as biotin and ligating the fragments to the marker, iv) purifying the marked fragments, v) adding labelled capture probes and selectively purifying the hybridized fragments, and vi) analyzing the fragments captured by hybridization and identify the fragments.
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