CHEMOENZYMATIC GLYCOENGINEERING OF ANTIBODIES AND FC FRAGMENTS THEREOF

摘要

The present invention provides for recombinant Endo-S mutants that exhibitreduced hydrolysis activity andincreased transglycosylation activity for the synthesis of glycoproteinswherein a desired sialylated oxazoline or syntheticoligosaccharide oxazoline is added to a core fucosylated or nonfucosylatedGlcNAc-protein acceptor. Such recombinant Endo-S mutants areuseful for efficient glycosylation remodeling of IgGl-Fc domain to providedifferent antibody glycoforms carrying structurallywell-defined Fc N-glycans.