METHOD OF SEPARATING AND PURIFYING ICARIIN FROM EPIMEDIUM EXTRACT

摘要

Disclosed is a method of separating and purifying icariin from Epimedium extract, relating to the technical field of separating and purifying a single component from a plant extract, and is intended to address the issues of complicated processing, high preparation costs, and environmental pollution in existing methods. The method comprises: 1) blending an Epimedium extract with an aqueous acetone solution having a volume fraction of 88%-96%; 2) filtering and collecting a filter cake, and then dissolving the filter cake in an aqueous ethanol solution having a volume fraction of 50%-75%; 3) after the dissolution is completed, filtering and collecting the filtrate, and concentrating the filtrate to remove the ethanol, thereby obtaining a concentrated liquid; 4) at a first temperature, adding acetone to the concentrated liquid, mixing uniformly, and lowering the temperature to a second temperature after sufficient dissolution to perform crystallization; 5) after the crystallization is completed, filtering and collecting a filter cake, and letting the filter cake dry to obtain crude icariin. The method can provide a single component having a icariin content of more than 98% that satisfies the high purity requirement of injection.