METHOD FOR SINGLE-CELL TRANSCRIPTOME AND ACCESSIBLE REGIONS SEQUENCING

摘要

The invention relates to a method for generating genome-wide accessible chromatin region and transcriptome information from a single cell comprising the steps of: (a) providing a nucleic acid sample from an individual cell, the nucleic acid sample comprising genomic DNA and messenger RNA (mRNA); (b) producing transposed DNA from the sample, preferably by treating the sample with Tn5 transposase and EDTA for inactivation of Tn5; (c) producing complementary DNA (cDNA) from the sample; (d) separating transposed DNA from cDNA; (e) amplifying transposed DNA, preferably by PCR; (f) amplifying cDNA, preferably by PCR and preparing a cDNA library from amplified cDNA; and (g) optionally sequencing transposed DNA and cDNA.