Method for constructing cell model for detecting pyrogen, cell model and pyrogen detection kit

摘要

A method for constructing a cell model for detecting a pyrogen, a cell model and a pyrogen detection kit. CRISPR/CAS9 is used to induce double bond breaks to form at specific positions of a genome, a homologous recombination is used to respectively knock TLR4 and CD14-MD2 into two chromosomes of a cell line, green fluorescent protein GFP and red fluorescent protein RFP are used for tracing, and finally a TLR4CD14/MD2 fixed-point knock-in fluorescent tracing cell model is successfully constructed.