The present subject matter relates to a device (10) and a method for digital holographic microscopy. The device (10) includes at least one light source (12) for illuminating an object (20), an optical element (22) for collecting an object light beam from the illuminated object (20), a condenser lens (112) arranged in a path of the object light beam and adapted for generating a condensed object light beam, and a beam divider (115) arranged in a path of the condensed object light for generating a first beam (I) and a second beam (II). The device (10) further includes a mask (141) for passage (142) of the first beam (I) and low-pass spatial filtering (144) the second beam (II), and an imaging unit (150) arranged for capturing an interference pattern generated by the first beam (I) and the second beam (II).
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