首页> 外国专利> METHOD FOR EVALUATING IMMUNITY ENHANCEMENT EFFECT OF IMMUNOGENIC MATERIAL BY USING DENDRITIC CELLS, AND METHOD FOR SCREENING FOR IMMUNOGENIC MATERIAL

METHOD FOR EVALUATING IMMUNITY ENHANCEMENT EFFECT OF IMMUNOGENIC MATERIAL BY USING DENDRITIC CELLS, AND METHOD FOR SCREENING FOR IMMUNOGENIC MATERIAL

机译:利用树突细胞评估免疫原性免疫增强效果的方法和筛选免疫原性材料的方法

摘要

The present invention relates to a method for evaluating an immunity enhancement or immunity improvement effect of an immunogenic material by using dendritic cells, and a method for screening for an immunogenic functional material and, more specifically, to a method for evaluating an immunity enhancement effect of an immunogenic material, comprising the steps of: (A) culturing a treatment group in which dendritic cells are treated with a target material extract, and a control group in which blanks are processed; (B) using flow cytometry so as to measure, in the treatment group and the control group, the cell ratio of a group (A0) in which MHC II is expressed but CD40 is not expressed, and a group (A1) in which MHC II is strongly expressed and CD40 is also expressed; and (C) digitizing an immunity enhancement effect of the target material according to the following formula.
机译:本发明涉及通过使用树突状细胞评价免疫原性材料的免疫增强或免疫改善效果的方法,以及筛选免疫原性功能材料的方法,更具体地,涉及用于评价树突状细胞的免疫增强功能的方法。一种免疫原性材料,包括以下步骤:(A)培养其中用靶材料提取物处理树突状细胞的处理组和其中处理空白的对照组。 (B)使用流式细胞术测定在治疗组和对照组中表达MHC II但不表达CD40的组(A0)和其中MHC的组(A1)的细胞比率II被强烈表达,CD40也被表达; (C)根据下式将靶材料的免疫增强效果数字化。

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