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METHOD FOR EVALUATING IMMUNITY ENHANCEMENT EFFECT OF IMMUNOGENIC MATERIAL BY USING DENDRITIC CELLS, AND METHOD FOR SCREENING FOR IMMUNOGENIC MATERIAL
METHOD FOR EVALUATING IMMUNITY ENHANCEMENT EFFECT OF IMMUNOGENIC MATERIAL BY USING DENDRITIC CELLS, AND METHOD FOR SCREENING FOR IMMUNOGENIC MATERIAL
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机译:利用树突细胞评估免疫原性免疫增强效果的方法和筛选免疫原性材料的方法
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摘要
The present invention relates to a method for evaluating an immunity enhancement or immunity improvement effect of an immunogenic material by using dendritic cells, and a method for screening for an immunogenic functional material and, more specifically, to a method for evaluating an immunity enhancement effect of an immunogenic material, comprising the steps of: (A) culturing a treatment group in which dendritic cells are treated with a target material extract, and a control group in which blanks are processed; (B) using flow cytometry so as to measure, in the treatment group and the control group, the cell ratio of a group (A0) in which MHC II is expressed but CD40 is not expressed, and a group (A1) in which MHC II is strongly expressed and CD40 is also expressed; and (C) digitizing an immunity enhancement effect of the target material according to the following formula.
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