A Method For Preparing Gold Nanorods Labeled With Schistosomiasis Antigen

摘要

#$%^&*AU2020100036A420200213.pdf#####Abstract The invention relates to a method for preparing gold nanorods labeled with Schistosomiasis antigen, which comprises the following steps: The spherical gold nanoparticles seeds are prepared by reducing HAuCl4 solution with a reducing agent; the spherical gold nanoparticles seeds are added into the growth medium to grow into rod-shaped gold nanorods in the rod micelle solution; the worm antigen labeled gold nanorods are obtained by using the Schistosomiasis adults antigen and / or the Schistosomiasis cercariae antigen. In the present invention, the "seed-mediated growth method" is selected to synthesize gold nanorods, so that the product yield and gold particle conversion rate are better than those prepared by the template method in which nucleation and growth are performed simultaneously; compared with the spherical-shape rods, the gold nanorods has obvious advantages in the aspect ratio. The results of the present invention are more sensitive and faster than ELISA. The present invention establishes a sensitive and rapid serological diagnosis technology for detecting schistosomiasis, which provides a simple and reliable clinical test technology for the serological diagnosis of schistosomiasis infection in early or mild infection.Aa AuC1 4 Reduing agent~ul u1+ CA A IAuC CI' Aut uI CAB bilayer 0Au Figurel %Vght No Akthtwig. "sidual Sum of Suwcs 0.01,,,. Pearson'o r 0,"792 Af~~R-Suare ."3775.)0- Vashe Standard Emvo. B Interept .5.019n 0.42072. ' 4.5 B If"p 0.01234 S.63940*4. 4.0,5- E=.S.019+.012*x (wavelength) 600 650 700 750 800 850 900 Figure2