ELISA kit for quantitative detection of content of insect cadherin and use method thereof

摘要

#$%^&*AU2020101140A420200730.pdf#####ABSTRACT The present invention discloses an ELISA kit for quantitative detection of content of insect cadherin and a use method thereof, and belongs to the technical field of biology. According to the present invention, recombinant cadherin is obtained by prokaryotic expression, a specific 5 monoclonal antibody is obtained by immunizing a rat with recombinant protein and screening, a specific polyclonal antibody is obtained by immunizing a rabbit with recombinant cadherin, finally an ELISA (enzyme linked immunosorbent assay) quantitative detection kit for quantitatively detecting content of cadherin is developed, and resistance of insects to Bt is determined by detecting the content of cadherin. The kit can be used for accurately and 0 reliably performing quantitative detection on cadherin, and judging the weakness of resistance of insects to Bt protein by detecting the content of cadherin. The kit is convenient and fast in detection and is good in stability. 14