A test kit for simultaneously detecting ten insect-borne disease pathogens and application thereof

摘要

#$%^&*AU2020102093A420201008.pdf#####ABSTRACT The invention provides a kit for simultaneously detecting ten insect-borne disease pathogens and application thereof, belonging to the technical field of liquid-phase chip detection. The test kit of the invention contains specific primers aiming at ten common insectborne disease pathogens and probes coupled with fluorescent labelled microspheres, and the nucleotide sequences of the specific primer pairs are shown as SEQ ID NO.1-20, and the nucleotide sequence of the probe is shown as SEQ ID NO.21-30. According to the test kit, by setting multiple asymmetric PCR systems and optimizing hybridization reaction conditions, the accurate detection of 10 kinds of insect-bome disease pathogens is realized. The test kit of the invention has the advantages of accurate detection, high sensitivity, strong specificity, simplicity and rapidness, which is suitable for port inspection and quarantine and epidemiological investigation of insect-borne diseases, and has a good application prospect.______________________________ RVFV(41 . sby(68) Averag *ebv(271 fluorescerice 4000 a BB (28) *asv(26) 2000 m ehdv (52) 1:1 1:2 1:3 1:4 1.5 SI(3 Proporion o fuptern~and dwteampinwr Figure 1 9000 Average ____ 64 fluorescence ii ~4 value 4WO ET *R 3C5I 3000 aEHDV{(52) 2000 mOF1(43) 1000 - BV(2 1) o URVFVI47) 0.5 1 2 4 6 1 fV(8 Di ffieer dos age of PRC pro ducts Figure 2