In vitro expression of pear PbrRALF2 protein and preparation method of polyclonal antibody thereof

摘要

#$%^&*AU2020102009A420201001.pdf#####ABSTRACT The invention discloses a pear PbrRALF2 protein expression in vitro and a preparation method of its polyclonal antibody, which comprises the following steps. Clone the PbrRALF2 gene of pear flower powder by designing primers; construct the recombinant expression vector PbrALF2-pCold-TF of Escherichia coli; transform the recombinant expression vector PbrALF2-pCold-TF into Escherichia coli BL21 (DE3) to construct the recombinant strain expressing pear PbrRALF2 protein; express he recombinant strain and purify it by nickel column affinity chromatography; use the purified recombinant pear PbrRALF2 protein as antigen to prepare pear PbrRALF2 polyclonal antibody by polyclonal antibody preparation method. This invention can realize the expression of pear PbrRALF2 protein in vitro, and prepare the pear PbrRALF2 polyclonal antibody with high titer and good specificity, which can fully meet the needs of related experiments and industrialization.