LATTICE LIGHT SHEET MICROSCOPE AND METHOD FOR TILING LATTICE LIGHT SHEET IN LATTICE LIGHT SHEET MICROSCOPE

摘要

The present disclosure relates to a lattice light sheet microscope and a method for tiling a lattice light sheet in the lattice light sheet microscope. The lattice light sheet microscope comprises: a single spatial light modulator (SLM) of which the optical modulation plane conjugates to an image plane of an excitation objective lens and which is configured to: generate an optical lattice by loading a phase map obtained from a central cross section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading a phase map obtained from an off-center cross section of the corresponding optical lattice; a transparent annular diaphragm, arranged at a plane conjugate to the entrance pupil of the excitation objective lens; and a first galvanometer, configured to: scan each optical lattice in the extending direction thereof so as to form a tiled lattice light sheet. Hence, an LLS may be easily tiled without changing the hardware structure of a conventional LLSM, and the high-resolution and high-speed imaging capabilities of an LLSM are maintained on an imaging field of view that is much greater than the size of the light sheet, which is convenient, low cost and may be easily applied.