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HIGHLY EFFICIENT METHOD FOR CATALYZING SYNTHESIS OF SANGUINARINE AND CHELERYTHRINE
HIGHLY EFFICIENT METHOD FOR CATALYZING SYNTHESIS OF SANGUINARINE AND CHELERYTHRINE
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机译:高效催化合成血红素和氯代酪氨酸的方法
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摘要
A highly efficient method for catalyzing the synthesis of sanguinarine and chelerythrine, comprising: from the known protopine-6-hydroxylase gene, dihydrobenzophenanthridine oxidase gene and cytochrome P450 reductase gene, by means of heterologous expression and result comparison and analysis, screening out an optimal gene having high expression efficiency, then performing codon optimization on the selected optimal gene; constructing the optimal gene sequence on an expression vector, then transferring same into yeast engineering bacteria for transformation so as to obtain recombinant yeast engineering strains; finally, feeding the recombinant yeast engineering bacteria by using a leaf raw material liquid precursor of Macleaya cordata for fermentation, so as to obtain a product. The invention improves the enzyme catalytic efficiency of sanguinarine and chelerythrine in various aspects such as gene level and fermentation process; the leaf raw material liquid, which is not a traditional medicinal part of Macleaya cordata, is directly used for fermentation with the engineering bacteria, and protopine and allocryptine having high alkaloid content in leaves are converted into high-value sanguinarine and chelerythrine to achieve the comprehensive utilization of Macleaya cordata resources.
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