EXTREMLY RAPID NUCLEIC ACID AMPLIFICATION METHOD, APPARATUS AND USE THEREOF

摘要

Disclosed is a method for detecting extremely rapid nucleic acid amplification, the method comprising repeatedly placing a nucleic acid amplification reaction tube in an ultra high temperature zone and an ultra low temperature zone for a short time, wherein the ultra high temperature zone is at a temperature point in the range of 100-150°C, and the ultra low temperature zone is at a temperature point in the range of 10-40°C; and performing such 30-40 cycles to complete the nucleic acid amplification detection process within 8-15 minutes. Also disclosed is a reactor for the extremely rapid nucleic acid amplification, the reactor comprising an ultra high temperature reaction zone with a fixed temperature in a regulation range of 100-150°C and an ultra-low temperature reaction zone with a fixed temperature in a regulation range of 10-40°C. Also disclosed is an operation software system and apparatus for the extremely rapid nucleic acid amplification.