DUCK PLAGUE VIRUS GE AND GI DUAL-GENE TRACELESS DELETION STRAIN DPV CHV-DELTA GE + DELTA GI AND CONSTRUCTION METHOD THEREFOR

摘要

Provided are a duck plague virus gE and gI dual-gene traceless deletion strain DPV CHv-delta gE + delta gI and a construction method therefor. A duck plague virus gE gene and a duck plague virus gI gene are deleted by means of two times of homologous recombination on a bacterial artificial chromosome recombination duck plague virus rescue system platform by using a GS1783 escherichia coli strain and a pEPkan-S plasmid, a MiniF element is deleted by using an intracellular spontaneous homologous recombination method, and thus the construction of a duck plague virus dual-gene traceless deletion strain free from exogenous base and MiniF element residue is completed for the first time.