Disclosed are a duck plague virus gC gene-deletion strain DPV CHv-ΔgC with a MiniF element removed and a construction method therefor. By using a GS1783 E. coli strain and a pEPKan-S plasmid, a MiniF element is deleted from a bacterial artificial chromosome recombinant duck plague virus rescuing system platform by means of an intracellular spontaneous homologous recombination method, such that the present invention finishes the construction of a duck plague virus traceless deletion strain without MiniF element residues for the first time, solves the problem that the MiniF element remains in a duck plague virus genome when a duck plague virus gene is deleted, and provides sufficient technical support for accurately exploring the functions of the duck plague virus gene and constructing attenuated live vaccines.
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