IMAGING SYSTEM OF STIMULATED EMISSION LOSS MICROSCOPE

摘要

An imaging system of a stimulated emission loss microscope, the system comprising a loss light unit (1), an excitation light unit (2), an imaging unit (3) and a counter unit (4). The loss light unit (1) generates ring loss light and controls the light intensity and polarization of the ring loss light; the excitation light unit (2) is triggered by the loss light unit (1) to emit Gaussian excitation light; the Gaussian excitation light is divided into first Gaussian excitation light and second Gaussian excitation light by means of a third Glan prism (243); the ring loss light overlaps the first Gaussian excitation light and then the overlapped light is inputted into the imaging unit (3); and the imaging unit (3) scans and images a sample and collects a fluorescent signal. The second Gaussian type excitation light is used as a reference signal and enters, together with the fluorescence signal, the counter unit (4), thereby being used for fluorescence intensity imaging and fluorescence lifetime imaging. When low ring loss light energy is applied, the fluorescence lifetime of the sample increases along with the laser irradiation time; and the saturation intensity of the fluorescent sample is reduced due to the increased fluorescence lifetime, so that the imaging resolution may be improved further.