Herein is reported a method for determining the dissociation rate constant kd of an antibody from its antigen at the dissociation pH-value, wherein the method comprises the steps of immobilizing at a first pH-value the antibody on a solid phase to which the antigen of the antibody has been conjugated; applying a pH-gradient from the first pH-value to the dissociation pH-value and thereafter maintaining the pH-value at said dissociation pH-value; and recording the binding signal during the maintaining of the pH-value and calculating therefrom the dissociation rate constant kd of the antibody from its antigen at the dissociation pH-value.
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机译：本文报道了一种在解离pH值下确定抗体与其抗原的解离速率常数k d 的方法，其中该方法包括将抗体固定在第一pH值下的步骤。抗体的抗原已经结合的固相;从第一pH值至解离pH值施加pH梯度，然后将pH值保持在所述解离pH值;在保持pH值的过程中记录结合信号，并由此计算在解离pH值下抗体从其抗原的解离速率常数k d 。
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