A METHOD FOR A FAST AND HIGH-QUALITY GENOMIC OR CELL-FREE DNA ISOLATION IN A SINGLE TUBE AND A KIT THEREFORE

摘要

The present invention is a method allowing intracellular genomic and cell-free DNA isolation via silica matrix in a single tube without performing the preliminary steps of the cell lysate or homogenate process, that are required to be applied prior to DNA isolation, and a kit therefore. It (kit) comprises a microcentrifuge tube (DNA-BM) that contains silica matrix, cell lysate, protein and lipid removers at appropriate concentrations for each DNA isolation and, mentioned DNA-BM contains DNA binding mix including Tris.HCI (Ph 6.5), EDTA, NaCL, Guanidum Thiocyanate, Nonidet P 40, Tween-20, SDS, DTT, Triton X100, silica beads.