HOMOLOGOUS RECOMBINATION EMPTY VECTOR FOR DUNALIELLA SALINA CHLOROPLASTS AND APPLICATION THEREOF

摘要

Provided are a homologous recombination empty vector for dunaliella salina chloroplasts and an application thereof. The vector comprises a promoter, a terminator, an upstream homologous arm having a base sequence represented by SEQ ID NO: 1, and a downstream homologous arm having a base sequence represented by SEQ ID NO: 2. A base sequence represented by SEQ ID NO: 5 that forms a polycistronic structure with at least one exogenous gene is inserted between the homologous arms. The vector can implement stable expression of multiple exogenous genes in chloroplasts.