GLUCOSE OXIDASE-BASED NANOCAPSULE SENSOR AND PREPARATION AND APPLICATION THEREOF

摘要

Enzyme-based biosensors have a broad range of potential for use in biomedicine, healthcare, food safety, environmental safety, and food fermentation, among other fields, and currently a main factor limiting commercialization thereof is the need to further increase the stability of enzyme sensors. Disclosed are a preparation method and an application of a highly stable enzyme sensor, and specifically a method of preparing an enzyme sensor on the basis of an enzyme molecule nanocapsule. The preparation of the enzyme nanocapsule mainly comprises the following steps: first preparing an enzyme single-molecule nanocapsule or an enzyme multi-molecule nanocapsule by means of in situ free radical polymerization, and then preparing the enzyme nanocapsule sensor with the enzyme molecule nanocapsule. The advantage of the enzyme sensor is that the thermal stability, organic solvent tolerance, and storage and service life thereof are significantly enhanced. The obtained nanocapsule sensor has a significant market application potential in human wearable devices, biomedicine, analytical testing, and food fermentation, among other fields.