Disclosed is a low-cost, scalable and highly repeatable approach to fabricate polystyrene films with three-dimensional nanopyramids on the surface. The nanopyramids have tubable aspect ratio and anti-bacterial performance. The effectiveness of the nanopyramids on bacterial and fungi growth inhibition and the role of nanostructure aspect ratio are confirmed via through scanning electron microscopy and confocal laser scanning microscopy. The results show an excellent antibacterial performance with more than 90%reduction in E. coli population in all nanopyramid samples after a 168-hr prolonged incubation time. The nanopyramid film developed here can be used for the clinical and commercial applications to prevent the growth of pathogenic bacteria on various surfaces.
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