METHOD FOR DETERMINING THE PERCENTAGE OF METHYLATION OF THE PROMOTER OF THE GENE O6-METHYLGUANINE-DNA METHYLTRANSFERASE (MGMT) IN CIRCULATING EXOSOMES

摘要

In the present invention a methodology has been identified for the detection of MGMT methylation in blood, serum or plasma of a human subject with high sensitivity and specificity, surpassing the techniques described so far. This methodology will allow the diagnosis and monitoring of those patients diagnosed with glioblastomas using a non-invasive method. This invention could be extended to other tumor types in which methylation of the MGMT gene was also present as a tumor marker.