PRODUCT FOR SCREENING LRPPRC REGULATORS AND METHOD FOR IDENTIFYING LRPPRC REGULATORS

摘要

Disclosed are a product for screening LRPPRC regulators and a method for identifying LRPPRC regulators, which product comprises a kit and a device, wherein the kit comprises a recombinant LRPPRC protein, GST protein, fluorescein-labeled aptamer, R14 and fluorescein-labeled control nucleic acid; and the device comprises a multi-hole plate and a fluorescence polarization signal detector. A short aptamer is used, which aptamer, in comparison with naturally occurring RNA and DNA sequences, has a shorter sequence and smaller molecular weight, which ensures that a polarization signal detection window is sufficiently large in a screening process, and a detection signal-to-noise ratio is successfully ensured; fine crystal structure analysis of the target protein is not needed in advance, which can greatly reduce a drug screening threshold; and the screening method has good universality for nucleic acid binding proteins and is easy to popularize.