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LASER-INDUCED CONFOCAL MICROSCOPE AND MICROFLUIDIC DEVICE FOR DIELECTROPHORETIC FLUORESCENCE-ACTIVATED DROPLET SORTING
LASER-INDUCED CONFOCAL MICROSCOPE AND MICROFLUIDIC DEVICE FOR DIELECTROPHORETIC FLUORESCENCE-ACTIVATED DROPLET SORTING
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机译:激光诱导的共焦显微和微流控装置,用于介电荧光激活的液滴分选
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摘要
Systems and method for sorting droplets includes a microfluidic chip or substrate having a droplet sorting channel coupled at an upstream location to a droplet inlet channel, the droplet sorting channel coupled at a downstream location to a waste channel and a collection channel. The device includes an optical interrogation device configured to illuminate the droplets passing through the sorting channel with excitation light from an excitation light source and capturing emitted fluorescent light and generating an output signal correlated to the fluorescence of the droplets. An actuator (electrode) is disposed in the microfluidic chip or substrate and coupled to a signal driver (e.g., a high voltage amplifier). The device or system uses a programmable controller configured to receive the output signals from the optical interrogation device and trigger the signal driver to actuate the actuator to direct the droplets into the collection channel.
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