Assays for Screening the Activity of Regulators of Members of the Hydroxysteroid (17-Beta) Dehydrogenase (HSD17B) Family

摘要

Kits are provided as well as screening methods to identify modulators of a hydroxysteroid (17-beta) dehydrogenase (HSD17B) family member protein, such as HSD17B13. The method includes HSD17B family inhibition, including inhibiting HSD17B family member protein as measured by substrate consumption, product concentration or NADH concentration from substrate conversion, HAD17B concentration, level of labeled substrate, luciferin light emission, or a combination thereof. Screening molecules for the ability to modulate family member proteins. Inhibitors of HSD17B family member proteins identified through screening methods can be used to treat liver diseases, disorders or conditions in which HSD17B family member proteins play a role.