Specific modification of plant non-coding RNA molecules to silence gene expression

摘要

Disclosed is a method for encoding a non-coding RNA molecule that does not have RNA silencing activity in a plant cell or modifying a gene processed into the non-coding RNA molecule. The method comprises introducing into the plant cell a DNA editing agent that confers silencing specificity of the non-coding RNA molecule towards a target RNA of interest. It's a way. Also disclosed are methods of encoding RNA silencing molecules in plant cells or modifying genes that are processed into the RNA silencing molecules. The method is a method comprising introducing a DNA editing agent into the plant cell that redirects the silent specificity of the non-coding RNA molecule toward the target RNA of interest. Plant cells, plant seeds, plants, and methods of producing plants are also disclosed.