The present invention relates to a method for determining the efficacy of DC, stimulating dendritic cells by incubation with soluble CD40L and TLR7/8 agonists, measuring secretion of marker proteins IL-10 and IL-12 from the stimulated dendritic cells Includes steps. This can determine whether dendritic cells have a large ability to activate T-cells and natural killer (NK) cells. The present invention also includes methods of stimulating dendritic cells, including stimulating dendritic cells with soluble CD40L and TLR7/8 agonists.
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