Nano-machined surface to capture cancer biomarkers

摘要

Herein, a cancer biomarker, such as a vesicle trapping surface, comprises: a substrate, a first plurality of nanoparticles attached to the substrate; A plurality of bifunctional tethers, wherein the first functionality of the bifunctional tether is attached to at least a portion of the first plurality of nanoparticles; A second plurality of nanoparticles attached to the second functionality of at least a portion of the plurality of bifunctional tethers; A capture agent attached to at least a portion of the second plurality of nanoparticles; And a plurality of polymer brush molecules attached to the surface, wherein the polymer brush molecule has a lower molecular weight than the bifunctional tether, and the polymer brush molecule reduces non-specific binding to the surface. . Also described is a method of capturing cancer biomarkers such as vesicles from a liquid biopsy sample.