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PRODUCTION AND PURIFICATION PROCESS OF HIGH PURITY RECOMBINANT HUMAN EPIDERMAL GROWTH FACTOR
PRODUCTION AND PURIFICATION PROCESS OF HIGH PURITY RECOMBINANT HUMAN EPIDERMAL GROWTH FACTOR
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机译:高纯度重组人表皮生长因子的生产及纯化工艺
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摘要
The present invention relates to a method for the production and purification of high purity recombinant human epithelial cell growth factors for purifying rhEGF with high purity through chromatography in a state where the purity of the rhEGF expressing protein is increased through a purification process without using a fusion protein. The method according to the present invention is characterized in that the recombinant rhEGF is purified with high purity by first washing, crushing and second washing the strain in a state in which recombinant rhEGF is expressed through transformation against E. coli BL21 (DE3).
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