UCST PARTICLE USING UCST AND METHOD FOR AMPLIFYING NUCLEIC ACID USING SAME

摘要

The present invention includes one or more primers and / or probes of forward and reverse primers in UCST (Upper Critical Solution Temperature) particles when amplifying a nucleic acid, or one or more of the forward and reverse primers and / or probes of UCST particles The primer or probe contained in the UCST particle can be released at a specific temperature range when nucleic acid amplification by immobilization on the hydrogel microparticles. Thus, it is possible to prevent the generation of primer duplexes and at the same time exhibit excellent PCR amplification efficiency.