The present invention relates to a live attenuated vaccine for the prevention of bovine coronavirus diarrhea occurring in calves and voice actors and a vaccination method using the same. In more detail, one domestic KWD20 isolate from Bovine coronavirus (BCoV), which is commonly used in Korea, was purified through 100 cell passages in HRT-18 cell lines, and cultured and purified from MDCK to 101 and 102 cells. The present invention relates to an attenuated mixed live vaccine containing a purified agent and a protective agent that minimizes virus titer loss and a vaccination method using the same.
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