3D Sea Anemone-derived Silk Protein-Based Bioink for 3D Bioprinting and Uses Thereof

摘要

The present invention relates to an anemone-derived silk protein-based 3D bio printing bio ink and its use, and more specifically, anemone-derived silk protein or a variant thereof; And a method of mixing and crosslinking the polymer to produce a 3D bio printer bio ink composition; A bio ink composition prepared according to the above method; And it provides a support for tissue engineering comprising the bio-ink composition. According to the present invention, the bio-ink composition of the present invention is capable of extrusion-based printing based on shear reduction characteristics, and can easily manufacture various types of three-dimensional structures through photocuring. The three-dimensional structure of the present invention can maintain its shape based on excellent physical properties, provides a bio-like environment for various cells, and provides excellent biocompatibility and biodegradability during biotransplantation, and thus is damaged in the field of tissue engineering and regenerative medicine. It can be used as a new platform for tissue replacement or artificial organ production for organ transplantation.