The present invention relates to a method for producing a DNA library based on an HiSeq sequencer for selective discovery of a mobile genetic factor SINE. On the basis of clinical and academic values of mobile genetic factors, the development of a next-generation base sequence decoding technology, the reduction of costs, and the development of an analysis system, a method for analyzing an AluYb8/9-specific base sequence which can identify human-specific AluYb8/9 is developed to be used as a method for simply and rapidly studying individual genetic variation by AluYb8/9, and genetic influences thereby. In addition, on the basis of the present invention, it is possible to develop additional technologies for targeting only active mobile genetic factors that show mobility in a human genome including AluYb8/9 and can cause genetic variation. A targeting sequence data analysis method as well as mobile genetic factor targeting base sequence analysis are generalized, and thus can be expected to be used in developing clinical medical diagnostic markers.
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