Medium composition for the differentiation of bile duct cells of liver stem cells and uses thereof

摘要

The present invention relates to a medium composition for inducing bile duct cell differentiation of liver stem cells and uses thereof, and more specifically, to provide a medium composition for differentiating liver stem cells into bile duct cells without going through an endoderm step, and the medium composition It relates to a method capable of rapidly obtaining bile duct cells by applying them during culture. According to the present invention, by adding a medium composition containing HGF, sodium taurocholate hydrate, and CHIR99021 in the process of culturing liver stem cells, bile duct cells can be quickly obtained without going through the endoderm step, When the bile duct cells are differentiated, gelatin is used as a cell support, so that clinically applicable bile duct cells can be obtained, which is expected to be useful in the treatment of cholangiopathy.