5' RNA Gene editing composition comprising sgRNAs with matched 5' nucleotide and gene editing method using the same

摘要

It is related to a technology for enhancing the efficiency of gene editing of a highly specific Cas9 variant using a guide RNA containing a matched 5'nucleotide, a complex of a guide RNA including a highly specific Cas9 variant and a matched 5'nucleotide, a high specificity Cas9 A composition for gene editing comprising a variant and a matched 5'nucleotide; And there is provided a gene editing method using the composition for gene editing.