3 Method for culturing 3D lung cancer organoid and preparing patient-derived xenograft model using thereof

摘要

The present invention relates to a three-dimensional lung cancer organoid culture method and a method for producing a patient-derived xenograft animal model using the same, and more specifically, the present invention is a three-dimensional lung cancer organoid culture method, a lung cancer organoid prepared by the method, the A medium composition for culturing lung cancer organoids, a method for preparing a xenograft animal model using cancer organoids, a patient-derived cancer organoid xenograft animal model prepared by the above method, a method for analyzing treatment efficacy of an anticancer agent using the animal model, and screening for anticancer agents It's about how. The three-dimensional lung cancer organoid culture method according to the present invention and the method of manufacturing a patient-derived xenograft animal model using the same can cultivate cancer tissues collected from cancer patients as three-dimensional organoids, and maintain the tissue and histological characteristics of the patient. At the same time, it can be cultured and transplanted while preserving the three-dimensional shape. In addition, the method for producing a patient-derived xenograft animal model according to the present invention can produce an animal model in a short time using a small amount of organoid cells compared to the conventional method. In addition, the method of manufacturing an animal model of the present invention not only has a high cancer xenotransplantation success rate, but can also be usefully used for research on specific cancer patients and screening for the most appropriate anticancer agent.