CAR-T transformation vector used for suppression of immune evasion through PDL1 blocking and its construction method and use

摘要

The present invention provides an AmpR sequence comprising an ampicillin resistance gene as shown in SEQ ID NO: 1; Prokaryotic replicon pUC Ori sequence as shown in SEQ ID NO: 2; Viral replicon SV40 Ori sequence as shown in SEQ ID NO: 3; EWPRE-enhanced prairie dog hepatitis B virus post-transcriptional regulatory element as shown in SEQ ID NO: 11; Human EF1α promoter as shown in SEQ ID NO: 12; A lentiviral packaging sheath element used for lentiviral packaging as shown in SEQ ID NO: 21; A humanized single chain antibody of human PDL1 that is PDL1scFv1, PDL1scFv2 as shown in SEQ ID NO: 22, or PDL1scFv3 as shown in SEQ ID NO: 23; An IRES ribosome binding sequence as shown in SEQ ID NO: 25; IL6 signal peptide as shown in SEQ ID NO: 26; Human antibody Fc fragment as shown in SEQ ID NO: 27; And a chimeric antigen receptor used in the composition of a second-generation CAR or a third-generation CAR that integrally recognizes, transfers, and operates a CAR-T transformation vector used for suppression of immune evasion through PDL1 blockade. The present invention provides a method for constructing the vector and the use of the vector in the manufacture of a drug for suppressing immune evasion.