首页> 外国专利> Manufacturing method of mutant strain having increased deinoxanthin productivity and the method for deinoxanthin overproduction by controlling cultivation temperature

Manufacturing method of mutant strain having increased deinoxanthin productivity and the method for deinoxanthin overproduction by controlling cultivation temperature

机译：脱黄嘌呤生产率提高的突变菌株的制备方法和通过控制培养温度脱黄嘌呤生产过剩的方法

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摘要

The present invention relates to a method for producing a mutant strain having high deinoxanthin production capability and a method for mass production of deinoxanthin using temperature control, and to a Deinococcus radiodurans having high deinoxanthin production capability prepared by the method of the present invention. The mutant strain does not have an artificially introduced antibiotic resistance gene even though it has been prepared by introducing a metabolic engineering method. In addition, the DR0862 ( crtB ) and DR1475 ( dxs ) genes are simultaneously inserted through the deinococcus genus mutant strain having a high-producing ability of deinoxanthin, without using an inducer such as IPTG, and by controlling the culture temperature, DR0862 And by controlling the expression of messenger RNA (mRNA) of the DR1475 gene, it is possible to increase the production of deinoxanthin, and thus, it can be usefully used in mass production of deinoxanthin having high antioxidant activity in a very economical way.

机译：本发明涉及具有高脱黄嘌呤生产能力的突变菌株的生产方法和利用温度控制大规模生产脱黄嘌呤的方法，并且涉及通过本发明的方法制备的具有高脱黄嘌呤生产能力的无核球菌。即使通过引入代谢工程方法制备了该突变株，也没有人工引入的抗生素抗性基因。另外，将DR0862（ crtB ）和DR1475（ dxs ）基因同时插入具有高产黄嘌呤黄嘌呤能力的脱奇球菌属突变株，而无需使用诱导剂例如IPTG，通过控制培养温度DR0862，并通过控制DR1475基因的信使RNA（mRNA）的表达，可以增加去黄嘌呤的产量，因此可用于大规模生产脱黄嘌呤以非常经济的方式具有高抗氧化活性。 展开▼

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公开/公告号KR102160203B1

专利类型

公开/公告日2020-09-25

原文格式PDF

申请/专利权人 UNIVERSITY OF SEOUL INDUSTRY COOPERATION FOUNDATION.;
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申请/专利号KR20200072482

发明设计人 최용준;정선욱;
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申请日2020-06-15

分类号C12N15/74;C12P23;

国家 KR

入库时间 2022-08-21 11:03:43

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