A method for the diagnosis of polymorphism of the NHLRC2 gene, causing a genetic defect in the duplication of the development of cattle of Aberdeen-Angus breed

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, in particular to methods for determining polymorphism of a gene associated with an undesirable recessive genetic duplication defect characteristic of cattle Aberdeen-Angus breed, specifically the NHLRC2 gene associated with the DDC haplotype. Method involves analysis of DNA extracted from biomaterial of animals by PCR method, characterized by that oligonucleotide primers of 20 bp are used for amplification of DNA fragment of mutation area. with melting point 59 °C and content of guanine-cytosine 55 %, forming in PCR amplicon length of 404 base pairs, which is then subjected to RFLP-analysis using enzyme BstMW I (restriction endonuclease) with recognition site GCNNNNN↑NNGC (CGNN↓NNNNNCG), which cuts out the PCR product in case of mutation on two DNA fragments - 320 base pairs and 84 base pairs.EFFECT: method is applicable in the farm animals genetics for detecting polymorphism in the Aberdeen-Angus cattle NHLRC2 gene associated with the genetic duplication defect (DD) carrier haplotype, in order to further use of obtained results in breeding and selection of cattle in breeding and commodity farms of meat cattle breeding industry.1 cl, 1 dwg, 1 ex