ELECTROCHEMICAL BIOSENSOR FOR DETERMINING BLOOD Glucose Concentration

摘要

The utility model relates to the technique of research and analysis of materials by determining electrochemical parameters and can be used to determine the concentration of glucose in biological solutions, for example, in blood. The technical result aimed at obtaining the utility model is to increase the accuracy of measuring the amount of glucose by a biosensor in the test material by increasing the production of electric current by a glucose biosensor. The technical result is achieved by the fact that an electrochemical biosensor is proposed for determining the concentration of glucose in the blood with an area for placement of the test material, made in the form of a substrate with measuring, reference and control electrodes applied on it, and the area for placement the test material is made in the form of a chamber formed between the specified substrate and located with a gap above it, a hydrophilic coating film, the gap between the substrate and the coating film is formed by adhesives hydrophobic gasket to them, made with a cutout above the measurement zone, the gasket being made in the form of a flat plate, while the measuring electrode, reference electrode and control electrode consist of two layers: the lower layer is made of copper, the upper layer is made of carbon and on top of the carbon layer of the measuring and reference electrodes locally above the measurement zone there is an electrobiochemical reagent made in the form of a homogeneous film consisting of 10% flavin-adenine dinucleotide-dependent glucose dehydrogenase, 70% potassium ferricyanide and 20% organic binder, obtained by drying in a convection chamber at 50 ° C, and the upper layers of the measuring and reference electrodes are in contact with the area of placement of the test material.