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SET OF SYNTHETIC OLIGONUCLEOTIDES FOR DETECTING DNAS OF REPRESENTATIVES OF DEER FAMILY

机译:用于检测鹿科家族代表DNA的合成寡核苷酸集

摘要

FIELD: biotechnology.;SUBSTANCE: invention refers to biotechnology, particularly to molecular genetics and genosystematics. Invention can be used to detect counterfeit derivatives (blood, horn, meat, and so forth) of deer and substitute them with other livestock products (chicken, cattle, pig, horse, small horned livestock); during meat components raw materials screening, for raw materials and biologically active substance detection, as well as for control over conformity of composition declared by the manufacturer. Primers and destructible probes are developed on the basis of data of species-specific nucleotide sequences of mitochondrial DNA of animals. Species-specific areas are represented by a mitochondrial cytochrome oxidase 1 (COX 1) fragment.;EFFECT: use of a set of synthetic oligonucleotides enables reliably identifying representatives of the deer family.;1 cl, 1 ex
机译:技术领域:发明:发明涉及生物技术,特别是分子遗传学和遗传系统学。发明可用于检测鹿的假冒衍生物(血液,牛角,肉等),并用其他牲畜产品(鸡,牛,猪,马,小角状牲畜)代替它们;在肉类成分原料筛选过程中,用于原料和生物活性物质检测以及控制制造商声明的成分符合性。引物和可破坏探针是根据动物线粒体DNA物种特异性核苷酸序列的数据开发的。物种特异性区域由线粒体细胞色素氧化酶1(COX 1)片段表示。效果:使用一组合成的寡核苷酸可以可靠地鉴定鹿类的代表。1cl,1 ex

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