METHOD OF ASSESSING THE EFFICIENCY OF TRANSPLANTATION OF FECAL MICROBIOTA IN PATIENTS WITH IMMUNE RESPONSE AFTER HAEMOPOETIC STEM CELL TRANSPLANTATION

摘要

FIELD: biotechnology.;SUBSTANCE: to this end, molecular-biological examination of a sample of fecal microbiota weighing 0.1 g on 30–45 days after FMT by identifying Bacteroides fragilis group by quantitative polymerase chain reaction of DNA recovered from a sample, using the following sequence of primers: forward: 5'-CGGAGGATCCGAGCGTTA-3'; reverse: 5'-CCGCAAACTTTCACAACTGACTTA-3'; fluorescent probe: 6 FAM-5'-CGCTCCCTTTAAACCCAATAAATCCGG-3'-BHQ-1, and when identifying Bacteroides fragilis group in amount of 10¹⁰ and more copies of gene in FMT sample is considered effective.;EFFECT: invention enables to obtain a threshold quantitative assessment of the Bacteroides fragilis group in a fecal microbiota sample for assessing the effectiveness of FMT, considerably simplifying the method and improving its accuracy.;1 cl, 5 dwg, 4 tbl