首页>
外国专利>
METHOD OF PRODUCING A CELLULAR MODEL FOR SARS-COV-2 VIRUS ANALYSIS
METHOD OF PRODUCING A CELLULAR MODEL FOR SARS-COV-2 VIRUS ANALYSIS
展开▼
机译:SARS-COV-2病毒分析细胞模型的产生方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
FIELD: biotechnology.;SUBSTANCE: invention represents a method involving culturing Caco2 cells on a semipermeable membrane insert in conditions of continuous current of the culture medium in a closed cycle for 7 days at the following parameters: pressure in the circuit from minus 12±25 % kPa to 12±25 % kPa, frequency of change of pressure from 4 to 5 Hz, difference of flow rate of culture medium from 0 to 0.9 mcl/s, average rate of culture medium current 5 mcl/min.;EFFECT: invention enables to obtain a differentiated monolayer of Caco2 cells with a physiological ratio of the expression of ACE2 and TMPRSS2 genesand can be used to produce a cell model based on differentiated human colon epithelial cells line Caco2 for studying SARS-CoV-2 virus.;1 cl, 2 ex
展开▼