Method for generating an overview image using a high aperture lens

摘要

The invention relates to a method for generating an overview image using a high aperture objective (4), comprising the steps of: providing an optical detection beam path (2) with the objective (4), the detection beam path (2) having a first numerical aperture in a first operating state ( NA1) and has a first depth of field (ST1), which lies essentially in a focal plane of the detection beam path (2) and, in the first operating state, an image of detection radiation with the first depth of field (ST1) and with a lateral image field is or are recorded The detection radiation is brought about by directing at least one light sheet (16) along an illumination axis (15) into a sample volume (8) to be imaged in the sample space; transferring the detection beam path (2) to a second operating state by the first depth of field ( ST1) to a second depth of field (ST2) wi rd, the high-aperture lens (4) remaining in the detection beam path (2), in the second operating state detecting a detection radiation, the detection radiation coming from a sample space being collected by the lens (2), guided along the detection beam path (2) and by means of at least one for the detector radiation-sensitive detector (7) is recorded as an image with a lateral image field, the lateral image fields being the same in the first and second operating states.