The invention relates to a method which makes it possible to transfer a radioisotope which is fixed on a first stationary phase contained in a first chromatography column to a second stationary phase contained in a second chromatography column to fix this radioisotope on the second stationary phase , and in which the radioisotope is chosen from the radioactive isotopes of thorium, radium, lead, bismuth and uranium. This method comprises at least the following steps: a) eluting the radioisotope from the first stationary phase with an aqueous solution A1 comprising an agent complexing the radioisotope, whereby an aqueous solution A2 is obtained which comprises complexes of the radioisotope; b) dissociation of the radioisotope complexes present in the aqueous solution A2 by modification of the pH of the aqueous solution A2, whereby an aqueous solution A3 is obtained comprising the decomplexed radioisotope; c) loading of the second stationary phase with the aqueous solution A3; and d) at least one washing of the second stationary phase with an aqueous solution A4.
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